LOCUS       pLV-[hsa-miR-1274a]        9155 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7076..7936
                     /label=Amp(R)
     promoter        8137..8677
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5681..5915
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6490..6945)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5987..6117
                     /label=SV40 pA
     misc_feature    6118..6331
                     /label=SV40 ori
     misc_feature    5002..5598
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3620
                     /label=intron
     CDS             3667..4992
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3410
                     /label=hsa-mir-1274a precursor
BASE COUNT     2154 a      2390 c      2452 g      2159 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccc cctacctatc 
     3121 tctatttgtc tctatctcta tttatctcta cttatttcta tttatcccta cttatctctg 
     3181 tttatccctg taggcctctt caggtccctt caggtctctt caggtctctg tttgtccctg 
     3241 atgtccctgt ttgtccctgt tcaggcgcca cctgtggctg tctgccacaa gtactatttg 
     3301 agaccatcac tacgacagtt actacttgag actgtcatta caagagttac ttctgttact 
     3361 acttgagacc atcattacag cagttactgc tgttactact tgagaccatc gctagctcga 
     3421 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     3481 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     3541 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     3601 gtttttttct tccatttcag gtgtcgtgaa aactacccct ctagagtcga gctaccggtc 
     3661 gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc 
     3721 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc 
     3781 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc 
     3841 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg 
     3901 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg 
     3961 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg 
     4021 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc 
     4081 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag 
     4141 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac 
     4201 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac 
     4261 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag 
     4321 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caaggaccca 
     4381 ccggtcgcca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 
     4441 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 
     4501 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 
     4561 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 
     4621 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 
     4681 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 
     4741 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 
     4801 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 
     4861 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 
     4921 accgtcaccg ccgacgtcga gtgcccgaag gaccgcgcga cctggtgcat gacccgcaag 
     4981 cccggtgcct gagcggccgc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat 
     5041 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 
     5101 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 
     5161 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 
     5221 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 
     5281 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 
     5341 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 
     5401 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 
     5461 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 
     5521 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 
     5581 gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc 
     5641 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 
     5701 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 
     5761 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 
     5821 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 
     5881 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 
     5941 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 
     6001 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 
     6061 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct 
     6121 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 
     6181 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 
     6241 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt 
     6301 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact 
     6361 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 
     6421 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 
     6481 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 
     6541 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 
     6601 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 
     6661 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 
     6721 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 
     6781 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 
     6841 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 
     6901 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg 
     6961 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 
     7021 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 
     7081 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 
     7141 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 
     7201 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 
     7261 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat 
     7321 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 
     7381 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 
     7441 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 
     7501 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 
     7561 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 
     7621 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 
     7681 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 
     7741 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 
     7801 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 
     7861 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 
     7921 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 
     7981 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 
     8041 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 
     8101 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 
     8161 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 
     8221 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca 
     8281 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 
     8341 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 
     8401 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 
     8461 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 
     8521 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 
     8581 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 
     8641 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 
     8701 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 
     8761 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac 
     8821 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg 
     8881 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 
     8941 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac 
     9001 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt 
     9061 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat 
     9121 taaccctcac taaagggaac aaaagctgga gctgc 
//
